Cholesterol determination using protein-templated fluorescent gold nanocluster probes.
نویسندگان
چکیده
We describe the development of a fluorescent biosensor platform for soluble cholesterol based on bovine serum albumin-stabilized gold nanocluster probes co-dissolved with cholesterol oxidase (ChOx) in a surfactant emulsion system. Selective enzymatic oxidation of cholesterol to cholest-4-en-3-one by ChOx produces stoichiometric amounts of H2O2 by-product, generating a quenching response signaling the presence of cholesterol at clinically relevant levels (LOD ∼12 μM).
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ورودعنوان ژورنال:
- The Analyst
دوره 138 24 شماره
صفحات -
تاریخ انتشار 2013